Malarial infection and curable sexually transmitted and reproductive tract infections (STIs/RTIs) are important
causes of adverse birth outcomes. Reducing the burden of these infections in pregnancy requires interventions
that can be easily integrated into the antenatal care (ANC) package. However, efforts to integrate the control of
malarial infection and curable STIs/RTIs in pregnancy have been hampered by a lack of evidence related to their
coinfection. Thus, we investigated the prevalence of coinfection among pregnant women of rural Zambia. A
prospective cohort study was conducted in Nchelenge District, Zambia, involving 1,086 first ANC attendees.
We screened participants for peripheral malarial infection and curable STIs/RTIs (syphilis, Chlamydia,
gonorrhea, trichomoniasis, and bacterial vaginosis), and collected relevant sociodemographic data at booking.
Factors associated with malarial and STI/RTI coinfection were explored using univariate and multivariate
regression models. Among participants with complete results (N = 1,071), 38.7% (95% confidence interval [CI]
= 35.7–41.6) were coinfected with malaria parasites and at least one STI/RTI; 18.9% (95% CI = 16.5–21.2)
were infected with malaria parasites only; 26.0% (95% CI = 23.5–28.8) were infected with at least one STI/RTI
but no malaria parasites, and 16.4% (95% CI = 14.1–18.6) had no infection. Human immunodeficiency virus
(HIV)-infected women had a higher risk of being coinfected than HIV-uninfected women (odds ratio [OR] =
3.59 [95% CI = 1.73–7.48], P < 0.001). The prevalence of malarial and STI/RTI coinfection was high in this
population. An integrated approach to control malarial infection and STIs/RTIs is needed to reduce this dual
burden in pregnancy.