The aim of the study was to develop guidelines for the assessment of abuse in women living with HIV/AIDS in Malawi. In phase one of the research, the experiences of these women were explored through a descriptive phenomenological study that involved 12 women living with HIV/AIDS who had encountered abuse. In phase 2, guidelines for the assessment of abuse were developed. Information from the interviews and a review of the literature was used to compile the draft guidelines. The guidelines were refined through a Delphi study carried out by nurses in the field of HIV/AIDS and gender-based violence. The guidelines could assist nurses to identify and address the abuse of women living with HIV/AIDS in a timely manner. Since the guidelines are inclusive of the actions nurses should take to encourage women to talk about their experiences, it is foreseen that their implementation could improve the quality of care rendered to the women.