Introduction: Family physician program as the greatest reforms of Iran's health system was initiated since 2008. The most important goals of this program were improvement in primary health services delivery. In this study, the Impact of family physician program implementation on the trend of changes in some of the maternal and newborn health indicators in a rural population of Isfahan province from 2001 to 2010 were investigated. Subject and Methods: This research was a retrospective study. The data were extracted from statistical and official documents. Indicators mean after and before family physician program implementation were compared using descriptive analysis and paired samples t-test. Results: The results of this study indicated that there were a significant difference between neonatal mortality rate, infant mortality rate and under-five mortality rate before and after of family physician program implementation. And performing this program has been the effective impact on improving mentioned indicators. The indicators of mothers mortality rate and low births weight rate were not indicated significant difference before and after of family physician program implementation. Conclusion: Considering trend of changes in studied indicators it can be said that family physician program implementation has been the effective impact on improving all of mother and child indicators except low births weight rate.