Enterococci abound in faeces, survive long outside the enteric environment and possess unique ability to
acquire and transfer antibiotic resistance. Recent studies have identified Enterococci as a relevant
indicator of water quality. The current study focused on the prevalence of antimicrobial resistant forms of
Enterococcus species isolated from Awedele spring water in Ekiti State Nigeria. The bacteriological and
physicochemical quality of the water samples was also assessed. The mineral content of the adjacent soil
samples was also determined. Antibiotic resistance among isolated Enterococcus species was highest to
penicillin as all isolates demonstrated complete resistance to the antibiotics. Prevalence of gentamycinresistant
enterococci was lowest (48%). Mean total bacterial and enterococcus count ranged from 1.8 x
10
4
to 8.6 x 10
4
cfu/ml and 1.5 x 10
5
to 4.0 x 10
5
cfu/ml respectively. While calling for an improved data
capturing system for drinking water surveillance in developing nations, the study highlights the need for
continuous efforts aimed at instigating the required hygiene behavioral change among residents of rural
settlements in the developing world.