The underdevelopment of the African pig industry is widely attributed to African swine fever (ASF). Outbreaks of the disease occur in different parts of Uganda almost annually although cases are rarely confirmed. We conducted an abattoir based survey of ASF associated lymph node lesions to establish the status of the disease in apparently healthy pigs. Highly suspicious lesions were subjected to immunohistochemistry for viral antigen detection. Most lymph nodes with follicular necrosis, parenchymal haemorrhage and lymphoid depletion were positive to ASF antigens. Up to 22 (0.1%) of the 258 pigs from which samples were collected were positive to ASF viral antigens. We conclude that domestic pigs in Uganda can act as reservoirs of the disease i.e. sustenance of the disease in pig populations may not be entirely dependent on the sylvatic cycle.