Context: Prenatal sex detection, allowing the selective abortions of female fetus is legally a penal offence. It is prevailing in many parts of India resulting in gender imbalance and high masculinity, because Indian value system prefers male child over female. Methods and Material: We interviewed 175 antenatal mothers by systemic random sampling, excluding the unwilling, attending OPD of Medical College and Hospital, Kolkata during January to June, to assess the trends in gender preference and awareness regarding pre-natal sex determination among antenatal women. Result: Our study couples, who are mostly from urban areas, mainly in the 20-30 year age group, mostly from lower economic strata, are well aware of the social menace arising out of prenatal sex determination and female feticide. Although one quarter had strong male preference, none undergone PCPNDT to know the sex of the offspring, 43% were aware that PCPNDT is legally banned and 54% knew that female feticide is a crime according to law. Conclusions: Provision and strict implementation of law as well as improvement of socio-economic condition, literacy through public education campaigns and lifting up the moral and ethical values are warranted to eradicate this social crime.