Introduction: Despite a dramatic progress in improvements regarding infant mortality due to HIV/AIDS in the
past decades, HIV/AIDS is causing a devastating impact on the world’s children. Mother-to-child transmission (MTCT) is
by far the largest source of HIV infection in children under the age of 15, with 90% of the cases infected during pregnancy,
birth, and major share is after birth.Therefore, this study follows infants after the first test (Deoxyribonucleic acid (DNA)
Polymerase Chain Reaction (PCR) testing) is negative, until the occurrences of HIV positivity and predictors associated
with it in the two hospital of Southern Ethiopia. Objective: The aim was to assess the incidence, Survival experiences and
predictors of HIV positivity among infants born from HIV positive mothers in two hospital of Southern Ethiopia, 2014.
Methodology: Institution-Based Retrospective follow up study was conducted at Hawassa Referral and Yirgalem General
Hospital from September 2000 to August 2005 E.C. Data was collected by trained BSc nurses from medical registration
book of 485 exposed infants . It was entered in to EPI Info 3.5.1 and exported to SPSS version 20 for further analysis. Both
Bivariate and Multiple variable Cox regression analysis were conducted to identify predictors. P-value< 0.05 was
considered as level of significance. Results: Among 457 participants included under analysis contributed for 4249.4 Person
Months of follow up. Cumulative and overall incidence rate of HIV positivity among infants were 19(4.16%) and
4.47/1000 PM (95% CI: 4.02-4.92) respectively. Mean HIV free survival time difference between Exclusive breast feeding
20.2 (95 % CI, 19.6, 20.8)), mixed feeding (17.5 (95% CI, 15.5-19.5)) and exclusive formula feeding 16.8 (95% CI, 16.3,
18.4) were significant. Mixed breast feeding (AHR: 8.23(1.98, 34.2)), ARV prophylaxis (yes) (AHR: 0.19(95% CI, 0.04-
0.89)), Maternal HAART (AHR: 0.16(95% CI, .041, 0.59) and SdNVP + AZT+ 3TC (AHR: 0.113(95% CI: 0.02, 0.61))
intervention were independent predictors of HIV positivity among exposed infants. Conclusion: The risk of mother to child
transmission of HIV is high for HIV exposed infants. All mothers should start PMTCT intervention as a guideline and it is
better to discourage mixed infant feeding to prevent the upcoming infants from the HIV infection.