Interviewer instructions
Conduct RDT TEST. RDT test results can be either Positive (GE+) meaning that the person has malaria parasites present in the blood or negative (GE-) meaning that the malaria parasites are absent. If GE-, record “2” and go to NEXT PERSON.
Testing for malaria is important not only so that those people that have malaria may be treated in the best way possible, but also so that further examinations may be carried out when a person does not test positive for malaria. In this way, RDTs can help to ensure appropriate treatment no matter what a sick person is suffering from. RDTs play an important role in diagnosing illnesses and can therefore be an important step in saving a person's life.
Procedure for testing Malaria using the Rapid Diagnostic Testing (RDT) method
As with the anemia test, the child should be sitting on the caregiver's lap, facing the RDT administrator (trained medical assistant). In the case of the eligible female household member, she should be sitting and facing the RDT administrator (trained medical assistant). To undertake the RDT test you will need the following supplies. New, unopened test packet (read expiry date); a cotton wool and sterilizing alcohol or methylated spirit); a new sterile lancet; a pair of disposable examination gloves; buffer; a timer (watch); a sharps disposal container and a non-sharps disposal bin.
Before testing for malaria, ask the household member if she (or child) has taken anti-malaria medication. If you are using an HRP2-detecting RDT: If the patient has taken a complete course of anti-malaria medication in the last 14 days, a positive RDT result may be misleading. It may be necessary to refer the patient to a health care center with a lab for further testing.
1. Put on a new pair of examination gloves.
2. Open the RDT box and get the test packet remove the contents-the teat cassette and the desiccant (the desiccant sachet that projects the test from humidity should be discarded),from the box, remove a blood transfer device- a pipette, an alcohol swab for cleaning the finger and a lancet for pricking
3. Write the patient's name on the cassette to avoid any confusion.
4. Open the alcohol swab and clean the patient's fourth or the third finger. If the patient is right-handed, use the patients' finger on the left hand. This will cause the least inconvenience to the patient if the pricked finger becomes sore. After cleaning the finger, it must be allowed to air dry. You should NOT blow on it or wipe it with a cloth or paper.
Once the finger is dry, open the lancet and prick the patient's finger towards the side of the pulp (ball) of the finger. Check to be sure the finger-prick will produce enough blood, then discard the lancet in the sharps container immediately. Never set the lancet down before discarding it. Never use a lancet on more than one person.
5. Collect the blood until the first mark.
6. Use the blood collection device pipette to add the drop of blood to the sample well (second well/small well). The blood needs to reach and be absorbed by the pad at the base of the hole
7. Discard the blood collection device after use into the sharp box immediately.
8. Add buffer to the cassette (round hole typically with the letter B). Make sure to count the correct number of drops. To do this, make sure to hold the bottle vertically.
9. Wait for 15 minutes after adding buffer before reading the test results. Write the time the results are going to be read on the test cassette.
10. Remove and discard your gloves.
11. Once the time has passed, first read the results yourself and do not tell the patients immediately. Reading the results:
12. The RDT cassette has two lines, the C- Control line and the line T-test line
a. A line in “T” and a line in “C” visible means the patient DOES have falciparum malaria. The test is positive even if the “T1” is very faint.
b. NO line in “T” but a line in “C” means the patient DOES NOT have either falciparum malaria..
c. No line in “T” and NO LINE in “C” means the test is damaged. Results are INVALID.
d. Line in “T” and NO LINE in “C” means the test is damaged. Results are INVALID.
13. Record the results and keep the used cassettes.